Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.

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Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

BiomaterialsElsevier,22, pp. Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering. From This Paper Figures, tables, and topics from this paper. Similar decreases in free primary amino group content and increases in thermal stability were obtained for collagen films treated with DPPA, glutaraldehyde, or hydrazine. Methods for the treatment of collagenous tissues for bioprostheses. Vacanti Journal of cellular biochemistry.

Have you forgotten your login? In order to validate and quantitate the extent of reaction, the degree of cross-linking was determined by the measure of the free primary amino group content of the samples. Glycosaminoglycans accumulated in the matrix, cllagen to 1. Application of Collagen Scaffold in Tissue Engineering: Monday, October 29, – 9: Showing of 71 extracted citations.

BonassarCharles A. Collagen-based biomaterials as 3D scaffold for cell cultures: Gelatine-Based Biomaterials and Their Biocompatibility: Both supports were always present during the study and a partial decrease in size and weight was detected only with control sponges, both seeded and unseeded.


Cytological evaluation of capsular tissue surrounding TCPL implant in adult rats. Showing of 84 references. AliDavid J Mooney Current topics in microbiology and immunology With the rapid development in tissue engineering, these different biomaterials have been used as three-dimensional scaffolds and cell collaen devices.

Collagen – References

Immunologically active biomaterials for cancer therapy. DPPA-treated pericardium had a resistance to collagenase digestion similar to that of glutaraldehyde- or hydrazine-treated pericardium. Howard The Journal of biological chemistry Cross-linking of collagen-based biomaterials increases their strength and persistence in vivo.

After determining the optimal conditions of cross-linking with DPPA, we compared the hrebage of this protocol with that using hydrazine and with the classical glutaraldehyde treatment. These in vitro results indicate that both collagen matrices can support the development of tissue engineered cartilage. References Publications referenced by this paper.

Collagen as matrix for neo-organ formation by gene-transfected fibroblasts. By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy PolicyTerms of Serviceand Dataset Collgen. In this report, we propose a simpler, faster way to prepare acyl azide groups and to cross-link collagen-based biomaterials, using diphenylphosphorylazide DPPA as a reagent.

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hrebage Daniel Herbage 1 AuthorId: The Perfect Match for Cardiac Repair? Cell proliferation was only noted in the 10 6 cells-seeded sponges 4-fold increase after 4 weeks of culture. Cross-linking of dermal sheep collagen using a collaven carbodiimide.

Wednesday, December 13, – 2: Aluminum Oxide Search for additional papers on this topic. Allogeneic Mesenchymal Stem Cells and Biomaterials: Topics Discussed in This Paper. Mineral deposition Glycosaminoglycan deposition Articular cartilage repair Collagen-based biomaterial Collagen synthesis. Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: Gelatinase A activation is regulated by the organization of the polymerized actin cytoskeleton.


Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

Collagen-based biomaterials in the form of sponges bovine type I collagen, both native and cross-linked by treatment with diphenylphosphorylazide, noted control ehrbage DPPA sponges respectively were tested as three-dimensional scaffolds to support chondrocyte proliferation with maintenance of the phenotype in order to form neocartilage.

Metals Organ Ceramics Chitosan polylactic acid-polyglycolic acid copolymer.

Development and characterisation of a three-dimensional in vitro hepatic fish model to investigate xenobiotic metabolism Matthew G Baron Citations Publications citing this paper. Anne-Marie Freyria herbafe AuthorId: Files produced by the author s. RosenthalGabriele J Koehler Anticancer research These results were corroborated by resistance to bacterial collagenase digestion: Chevallay and Dr D.

Specific cartilage collagens types II and XI were deposited in the matrix throughout the culture and hebage of type I collagen were noticed only in the culture medium after weeks and 4 weeks in the case of 10 6 and 10 7 cells-seeded sponges, respectively.

ChevallayDr D. Ham A Benghuzzi Biomedical sciences instrumentation